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PurposeMammalian expression of human JMJD2C with HA tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGASC1
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Alt namejmjd2c
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SpeciesH. sapiens (human)
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Insert Size (bp)4000
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Entrez GeneKDM4C (a.k.a. GASC1, JHDM3C, JMJD2C, TDRD14C)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Bglob-intron-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CASC1 has several point mutations - D486G E633D and K772R.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-HA-GASC1 was a gift from Kristian Helin (Addgene plasmid # 24214 ; http://n2t.net/addgene:24214 ; RRID:Addgene_24214) -
For your References section:
The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3. Cloos PA, Christensen J, Agger K, Maiolica A, Rappsilber J, Antal T, Hansen KH, Helin K. Nature. 2006 Jul 20. 442(7100):307-11. 10.1038/nature04837 PubMed 16732293
