pCMV-HA-JMJD2B
(Plasmid #24181)

• Purpose: Mammalian expression of human JMJD2B with HA tag
• Depositing Lab: Kristian Helin
• Publication: Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JMJD2B
• Species: H. sapiens (human)
• Insert Size (bp): 4000
• Entrez Gene: KDM4B (a.k.a. JMJD2B, MRD65, TDRD14B)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Bglob-intron-F

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains S598P and E738K within the JMJD2B insert. Please note that it is unclear whether these mutations affect plasmid function.