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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24179 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti6/UbC/V5-Dest
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 9400
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Vector typeMammalian Expression, Lentiviral ; Gateway
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsStbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameChloramphenicol resistance gene (CmR) - ccdB gene
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SpeciesChloramphenicol resistance gene (CmR) - ccdB gene
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Tag
/ Fusion Protein
- Myc (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer sequence
- 3′ sequencing primer sequence (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInvitrogen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
V5 tag deleted
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVU/myc was a gift from Lars Ittner (Addgene plasmid # 24179 ; http://n2t.net/addgene:24179 ; RRID:Addgene_24179)