pLVU/RED
(Plasmid #24178)

• Depositing Lab: Lars Ittner
• Publication: Krupka et al Plasmid. 2010 Feb 2. ():.

Backbone

• Vector backbone: pLenti6/UbC/V5-Dest
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 9956
• Vector type: Mammalian Expression, Lentiviral ; Gateway
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: ccdB survival strain
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Chloramphenicol resistance gene (CmR) - ccdB gene
• Species: Chloramphenicol resistance gene (CmR) - ccdB gene
• Tag / Fusion Protein:
  • DsRED (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attR1 (destroyed during cloning)
• 3′ cloning site: attR2 (destroyed during cloning)
• 5′ sequencing primer: sequence
• 3′ sequencing primer: sequence

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Invitrogen

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

V5 tag deleted