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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLenti6/UbC/V5-Dest
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 9998
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Vector typeMammalian Expression, Lentiviral ; Gateway
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdB survival strain
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameChloramphenicol resistance gene (CmR) - ccdB gene
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SpeciesChloramphenicol resistance gene (CmR) - ccdB gene
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Tag
/ Fusion Protein
- eGFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (destroyed during cloning)
- 3′ cloning site attR2 (destroyed during cloning)
- 5′ sequencing primer sequence
- 3′ sequencing primer sequence (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInvitrogen
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
V5 tag deleted
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVU/GFP was a gift from Lars Ittner (Addgene plasmid # 24177) -
For your References section:
Gateway-compatible lentiviral transfer vectors for ubiquitin promoter driven expression of fluorescent fusion proteins. Krupka N, Strappe P, Götz J, Ittner LM. Plasmid. 2010 Feb 2. ():. 10.1016/j.plasmid.2010.01.002 PubMed 20132838