pIRES.Puro.EGFP
(Plasmid #24176)

• Depositing Lab: Robert Sobol
• Publication: Jelezcova et al Mutat Res. 2010 Jan 22. ():.

Backbone

• Vector backbone: pIRESpuro-modified with gateway cassette by recombination clonin
• Backbone manufacturer: Clontech (Unmodified)
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP
• Alt name: X
• Insert Size (bp): 720
• Mutation: x
• GenBank ID: NC_013179

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: x (destroyed during cloning)
• 3′ cloning site: x (destroyed during cloning)
• 5′ sequencing primer: X
• 3′ sequencing primer: x

Resource Information

• A portion of this plasmid was derived from a plasmid made by: EGFP was cloned from pIRES-EGFP)Clontech)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

EGFP was cloned into pENTR-D (invitrogen). The EGFP open reading frame was transferred from pENTRD- EGFP to a gateway modified pIRES-Puro plasmid via LR recombination reaction.