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Addgene

pIRES.Puro.mBeta
(Plasmid #24175)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro-modified with gateway cassette by recombination clonin
  • Backbone manufacturer
    Clontech (Unmodified)
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pIRES.Puro.mBeta
  • Alt name
    Mouse Polymerase Beta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1008
  • Mutation
    x
  • GenBank ID
    NM_011130
  • Entrez Gene
    Polb (a.k.a. A430088C08Rik)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site x (not destroyed)
  • 3′ cloning site x (not destroyed)
  • 5′ sequencing primer x
  • 3′ sequencing primer x
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    x

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mouse pol beta cDNA was cloned into pENTR-D (invitrogen). The mouse pol beta open reading frame was transferred from pENTRD-mPOL beta to a gateway modified pIRES-Puro plasmid via LR recombination reaction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES.Puro.mBeta was a gift from Robert Sobol (Addgene plasmid # 24175 ; http://n2t.net/addgene:24175 ; RRID:Addgene_24175)

  • For your References section:

    Parp1 activation in mouse embryonic fibroblasts promotes Pol beta-dependent cellular hypersensitivity to alkylation damage. Jelezcova E, Trivedi RN, Wang XH, Tang JB, Brown AR, Goellner EM, Schamus S, Fornsaglio JL, Sobol RW. Mutat Res. 2010 Jan 22. ():. 10.1016/j.mrfmmm.2010.01.016 PubMed 20096707
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