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Addgene

pLLOXmPOLBeta
(Plasmid #24174)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLentiLox3.7
  • Backbone manufacturer
    Obtained from MIT vector core
  • Backbone size w/o insert (bp) 70000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pLLOXmPOLbeta
  • Alt name
    Mouse Polymerase Beta shRNA
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    65
  • Mutation
    x
  • GenBank ID
    NM_011130
  • Entrez Gene
    Polb (a.k.a. A430088C08Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (destroyed during cloning)
  • 3′ cloning site XnoI (destroyed during cloning)
  • 5′ sequencing primer X
  • 3′ sequencing primer X
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Backbone obtained from MIT vector core

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

shRNA oligo sequence is - 5'-TGATCAGTACTACTGTGGTGTTCAAGAGACACCACAGTAGTACTGATCTTTTTTC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLLOXmPOLBeta was a gift from Robert Sobol (Addgene plasmid # 24174 ; http://n2t.net/addgene:24174 ; RRID:Addgene_24174)

  • For your References section:

    Parp1 activation in mouse embryonic fibroblasts promotes Pol beta-dependent cellular hypersensitivity to alkylation damage. Jelezcova E, Trivedi RN, Wang XH, Tang JB, Brown AR, Goellner EM, Schamus S, Fornsaglio JL, Sobol RW. Mutat Res. 2010 Jan 22. ():. 10.1016/j.mrfmmm.2010.01.016 PubMed 20096707
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