pCMV-HA-JMJD3
(Plasmid #24167)

• Purpose: Mammalian expression of human JMJD3 with HA tag
• Depositing Lab: Kristian Helin
• Publication: Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

Backbone

• Vector backbone: pCMV
• Backbone size w/o insert (bp): 6600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JMJD3
• Species: H. sapiens (human)
• Insert Size (bp): 5400
• Entrez Gene: KDM6B (a.k.a. JMJD3, NEDCFSA, NEDSST)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Bglob-intron-F

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains EAW90127.1 with an E433K mutation.