CLM RP KGF Mut 1
(Plasmid
#24166)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET17b
- Backbone size w/o insert (bp) 3300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKinesin
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Alt nameK560CLM-GFP
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Alt nameKIF5B
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SpeciesH. sapiens (human)
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Insert Size (bp)1680
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MutationC7S/C65A/C168A/C174S/C294A/C330S/C421A
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Entrez GeneKIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
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Tags
/ Fusion Proteins
- GFP (C terminal on insert)
- 6XHis (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer T7, GFP-R
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CLM RP KGF Mut 1 was a gift from Ron Vale (Addgene plasmid # 24166 ; http://n2t.net/addgene:24166 ; RRID:Addgene_24166) -
For your References section:
Controlling kinesin by reversible disulfide cross-linking. Identifying the motility-producing conformational change. Tomishige M, Vale RD. J Cell Biol. 2000 Nov 27. 151(5):1081-92. 10.1083/jcb.151.5.1081 PubMed 11086009