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Addgene

GFP-PK
(Plasmid #24163)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pyruvate kinase
  • Alt name
    PK
  • Species
    G. gallus (chicken)
  • Mutation
    n/a
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-PK was a gift from Warner Greene (Addgene plasmid # 24163 ; http://n2t.net/addgene:24163 ; RRID:Addgene_24163)

  • For your References section:

    Nucleocytoplasmic shuttling by human immunodeficiency virus type 1 Vpr. Sherman MP, de Noronha CM, Heusch MI, Greene S, Greene WC. J Virol. 2001 Feb . 75(3):1522-32. 10.1128/JVI.75.3.1522-1532.2001 PubMed 11152524
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