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Addgene

T7-RelA(S276E)
(Plasmid #24154)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pEV3S (pLG-033)
  • Backbone manufacturer
    Matthias et al, Nucleic Acid Research (1989).
  • Backbone size w/o insert (bp) 5120
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RelA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    S276E
  • Entrez Gene
    RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
  • Tag / Fusion Protein
    • T7 gp10 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FOr more details for this expression construct as well as other mutants, please contact Dr Lin-Feng CHen ([email protected])

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-RelA(S276E) was a gift from Warner Greene (Addgene plasmid # 24154 ; http://n2t.net/addgene:24154 ; RRID:Addgene_24154)

  • For your References section:

    NF-kappaB RelA phosphorylation regulates RelA acetylation. Chen LF, Williams SA, Mu Y, Nakano H, Duerr JM, Buckbinder L, Greene WC. Mol Cell Biol. 2005 Sep . 25(18):7966-75. 10.1128/MCB.25.18.7966-7975.2005 PubMed 16135789
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