pLKO.1 hygro
(Plasmid #24150)

• Purpose: (Empty Backbone) 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
• Depositing Lab: Bob Weinberg
• Publication: empty vector (unpublished)

Backbone

• Vector backbone: pLKO.1 - TRC cloning vector (no stuffer)
• Backbone manufacturer: David Root
• Backbone size (bp): 7500
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: U6 for shRNA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: LKO1_5
• 3′ sequencing primer: SV40pA-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 59 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is similar to the pLKO.1-TRC cloning vector (www.addgene.org/10878) except the stuffer between AgeI and EcoRI is only about 24 bp (instead of 1.7kb) and the puro gene has been replaced by hygro resistance.

Information for Cloning Grade DNA (Catalog # 24150-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here