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Addgene

PCMV4-3 HA/IkB-alpha(SS32,36AA)
(Plasmid #24143)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24143 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV4-3xHA
  • Backbone manufacturer
    Green lab
  • Backbone size w/o insert (bp) 4874
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IkB alpha
  • Species
    H. sapiens (human)
  • Mutation
    S32A S36A
  • Entrez Gene
    NFKBIA (a.k.a. EDAID2, IKBA, MAD-3, NFKBI)
  • Tag / Fusion Protein
    • 3xHA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCMV4-3 HA/IkB-alpha(SS32,36AA) was a gift from Warner Greene (Addgene plasmid # 24143 ; http://n2t.net/addgene:24143 ; RRID:Addgene_24143)

  • For your References section:

    Both amino- and carboxyl-terminal sequences within I kappa B alpha regulate its inducible degradation. Sun S, Elwood J, Greene WC. Mol Cell Biol. 1996 Mar . 16(3):1058-65. PubMed 8622650
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