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Addgene

p6268 pDEST-59-His-Trx-HPV16E6
(Plasmid #24126)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24126 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDEST-59
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7198
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HPV16 E6
  • Species
    HPV
  • Insert Size (bp)
    470
  • Mutation
    none
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)
  • Entrez Gene
    E6 (a.k.a. HpV18gp1)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Trx (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attR1 (unknown if destroyed)
  • 3′ cloning site attR2 (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p6268 pDEST-59-His-Trx-HPV16E6 was a gift from Peter Howley (Addgene plasmid # 24126 ; http://n2t.net/addgene:24126 ; RRID:Addgene_24126)