pK.myc-MYPT1
(Plasmid #24101)

• Depositing Lab: Masumi Eto
• Publication: Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2):100-9.

Backbone

• Vector backbone: pKmyc
• Backbone manufacturer: Addgene plasmid 19400
• Backbone size w/o insert (bp): 4200
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myosin-targeting subunit-1
• Alt name: MBS
• Alt name: MYPT1
• Alt name: M110
• Species: H. sapiens (human)
• Insert Size (bp): 3000
• Mutation: S50G
• GenBank ID: AF458589
• Entrez Gene: PPP1R12A (a.k.a. GUBS, M130, MBS, MYPT1)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry