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Addgene

GFP-DRD4
(Plasmid #24100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24100 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1+/Hygro
  • Backbone size w/o insert (bp) 5601
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DRD4
  • Species
    H. sapiens (human)
  • Entrez Gene
    DRD4 (a.k.a. D4DR)
  • Tags / Fusion Proteins
    • alpha7 nicotinic signal peptide (N terminal on backbone)
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-DRD4 was a gift from Jean-Michel Arrang (Addgene plasmid # 24100)

  • For your References section:

    Interactions of GIPC with dopamine D2, D3 but not D4 receptors define a novel mode of regulation of G protein-coupled receptors. Jeanneteau F, Diaz J, Sokoloff P, Griffon N. Mol Biol Cell. 2004 Feb . 15(2):696-705. 10.1091/mbc.e03-05-0293 PubMed 14617818
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