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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24098 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCEP4
- Backbone size w/o insert (bp) 10200
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDRD3
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SpeciesH. sapiens (human)
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Entrez GeneDRD3 (a.k.a. D3DR, ETM1, FET1)
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Tags
/ Fusion Proteins
- alpha7 nicotinic signal peptide (N terminal on backbone)
- eGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-DRD3 was a gift from Jean-Michel Arrang (Addgene plasmid # 24098 ; http://n2t.net/addgene:24098 ; RRID:Addgene_24098) -
For your References section:
Interactions of GIPC with dopamine D2, D3 but not D4 receptors define a novel mode of regulation of G protein-coupled receptors. Jeanneteau F, Diaz J, Sokoloff P, Griffon N. Mol Biol Cell. 2004 Feb . 15(2):696-705. 10.1091/mbc.e03-05-0293 PubMed 14617818
Map uploaded by the depositor.
