HSD2-FLAG
(Plasmid #24097)

• Depositing Lab: Moses Chao
• Publication: Jeanneteau et al Proc Natl Acad Sci U S A. 2008 Mar 25. 105(12):4862-7.

Backbone

• Vector backbone: FCIV1
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HSD2
• Alt name: 11beta hydroxysteroid dehydrogenase
• Species: H. sapiens (human)
• Insert Size (bp): 1250
• Entrez Gene: HSD11B2 (a.k.a. AME, AME1, HSD11K, HSD2, SDR9C3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI/NheI (unknown if destroyed)
• 5′ sequencing primer: TRC-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HSD2-flag cDNA sequences was subcloned into FCIV1 lentiviral vector. The venus is not fused to the HSD2 and expression of venus is driven by an IRES sequence.