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Addgene

HSD11B1-FLAG
(Plasmid #24096)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24096 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FCIV1
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HSD11beta1
  • Alt name
    11b hydroxysteroid 1b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    876
  • GenBank ID
    AK313973
  • Entrez Gene
    HSD11B1 (a.k.a. 11-DH, 11-beta-HSD1, CORTRD2, HDL, HSD11, HSD11B, HSD11L, SDR26C1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NheI (destroyed during cloning)
  • 5′ sequencing primer TRC-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HSD1-flag cDNA sequences was subcloned into FCIV1 lentiviral vector. The venus is not fused to the HSD1 and expression of venus is driven by an IRES sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HSD11B1-FLAG was a gift from Moses Chao (Addgene plasmid # 24096 ; http://n2t.net/addgene:24096 ; RRID:Addgene_24096)

  • For your References section:

    Activation of Trk neurotrophin receptors by glucocorticoids provides a neuroprotective effect. Jeanneteau F, Garabedian MJ, Chao MV. Proc Natl Acad Sci U S A. 2008 Mar 25. 105(12):4862-7. 10.1073/pnas.0709102105 PubMed 18347336
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