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Addgene

pcDNA HA HP1 gamma delta C
(Plasmid #24081)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HP1 gamma
  • Species
    H. sapiens (human)
  • Mutation
    Missing C-terminal 25 amino acids
  • Entrez Gene
    CBX3 (a.k.a. HECH, HP1-GAMMA, HP1Hs-gamma, HP1gamma)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA HA HP1 gamma delta C was a gift from Naoko Tanese (Addgene plasmid # 24081 ; http://n2t.net/addgene:24081 ; RRID:Addgene_24081)

  • For your References section:

    Isoform-specific interaction of HP1 with human TAFII130. Vassallo MF, Tanese N. Proc Natl Acad Sci U S A. 2002 Apr 30. 99(9):5919-24. 10.1073/pnas.092025499 PubMed 11959914
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