pcDNA HA HP1 alpha
(Plasmid #24078)

• Depositing Lab: Naoko Tanese
• Publication: Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 99(9):5919-24.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HP1 alpha
• Species: H. sapiens (human)
• Entrez Gene: CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry