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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4T-1
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHP1 alpha
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SpeciesH. sapiens (human)
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Entrez GeneCBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST HP1 alpha was a gift from Naoko Tanese (Addgene plasmid # 24074 ; http://n2t.net/addgene:24074 ; RRID:Addgene_24074) -
For your References section:
Isoform-specific interaction of HP1 with human TAFII130. Vassallo MF, Tanese N. Proc Natl Acad Sci U S A. 2002 Apr 30. 99(9):5919-24. 10.1073/pnas.092025499 PubMed 11959914