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Addgene

pBSCSX
(Plasmid #24072)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD33
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CLiPS caspase-3 substrate
  • Insert Size (bp)
    600

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer see map
  • 3′ sequencing primer see map
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSCSX was a gift from Patrick Daugherty (Addgene plasmid # 24072 ; http://n2t.net/addgene:24072 ; RRID:Addgene_24072)

  • For your References section:

    Protease specificity determination by using cellular libraries of peptide substrates (CLiPS). Boulware KT, Daugherty PS. Proc Natl Acad Sci U S A. 2006 May 16. 103(20):7583-8. 10.1073/pnas.0511108103 PubMed 16672368
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