-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backboneMSCV-LTRmiR30-PIG (LMP)
-
Backbone manufacturerOpen Biosystems
- Backbone size w/o insert (bp) 7894
-
Vector typeRetroviral, RNAi
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameFoxp3
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)21
-
Entrez GeneFoxp3 (a.k.a. JM2, scurfin, sf)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV-LMP-F (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The target sequence of Foxp3: 5′-GGCAGAGGACACTCAATGAAAT-3′
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
LMP 1066 was a gift from Dan Littman (Addgene plasmid # 24071 ; http://n2t.net/addgene:24071 ; RRID:Addgene_24071) -
For your References section:
TGF-beta-induced Foxp3 inhibits T(H)17 cell differentiation by antagonizing RORgammat function. Zhou L, Lopes JE, Chong MM, Ivanov II, Min R, Victora GD, Shen Y, Du J, Rubtsov YP, Rudensky AY, Ziegler SF, Littman DR. Nature. 2008 May 8. 453(7192):236-40. 10.1038/nature06878 PubMed 18368049