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Addgene

MIGR-IL23R
(Plasmid #24066)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24066 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIGR
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IL23R
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2000
  • Entrez Gene
    Il23r (a.k.a. IL-23R)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HpaI (destroyed during cloning)
  • 5′ sequencing primer pLXSN 5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

IL23R cDNA amplified through IL6 treated cDNA as 2 parts (5' part ATG-134-BamHI; 3' part 134-STOP).
5' part cloned into pcDNA3 XhoI/BamHI
3' part cloned into MSCV hCD2
Part linked together in pcDNA3 (XhoI-MluI)
Then cut with XhoI/NruI cloned into pMIGR (XhoI/HpaI)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIGR-IL23R was a gift from Dan Littman (Addgene plasmid # 24066 ; http://n2t.net/addgene:24066 ; RRID:Addgene_24066)
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