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Addgene

RSV-Pit1
(Plasmid #24059)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSV
  • Backbone size w/o insert (bp) 7000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pit1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1000
  • Entrez Gene
    SLC20A1 (a.k.a. GLVR1, Glvr-1, PIT1, PiT-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site Kpn I (unknown if destroyed)
  • 5′ sequencing primer pREP-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RSV-Pit1 was a gift from Herbert Samuels & Frederick Stanley (Addgene plasmid # 24059 ; http://n2t.net/addgene:24059 ; RRID:Addgene_24059)

  • For your References section:

    The homeodomain protein, Pit-1/GHF-1, is capable of binding to and activating cell-specific elements of both the growth hormone and prolactin gene promoters. Fox SR, Jong MT, Casanova J, Ye ZS, Stanley F, Samuels HH. Mol Endocrinol. 1990 Jul . 4(7):1069-80. 10.1210/mend-4-7-1069 PubMed 2284007
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