pVitro2-hygro-N-myc-hVps34/hVps15-C-V5-his
(Plasmid #24055)

• Depositing Lab: Jonathan Backer
• Publication: Yan et al Biochem J. 2009 Feb 1. 417(3):747-55.

Backbone

• Vector backbone: pVitro2
• Backbone manufacturer: Invivogen
• Backbone size w/o insert (bp): 6321
• Vector type: Mammalian Expression ; bicistronic
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: may need up to 2 days to grow
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PIK3C3 and PIK3R4
• Alt name: hVps34 and hVps15
• Species: H. sapiens (human)
• Insert Size (bp): 2700
• Entrez Gene: PIK3C3 (a.k.a. VPS34, Vps34, hVps34)
• Entrez Gene: PIK3R4 (a.k.a. VPS15, p150)
• Tags / Fusion Proteins:
  • Myc (hVps34) (N terminal on insert)
  • V5/his (hVps15) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (hVps34); NheI (hVps15) (not destroyed)
• 3′ cloning site: AvrII (hVps34); NheI (hVps15) (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ludwig Institute for Cancer Research
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry