HIR Y1158F
(Plasmid #24051)

• Depositing Lab: Frederick Stanley
• Publication: Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1):7-16.

Backbone

• Vector backbone: pRT3
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Insulin Receptor
• Species: H. sapiens (human)
• Insert Size (bp): 4600
• Mutation: Y1158F
• Entrez Gene: INSR (a.k.a. CD220, HHF5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The amino acid assignments for the mutations come from an alternate intron that shifts the location of these sites so the K1030R corresponds to other mutations made by others that are K1018R. The clone of the receptor came from William Rutter (Cell 45, 721-732, 1986) who has the same numbering as the Stanley lab. Other researchers do not have the 12 aa alternate intron, and the relevancy of this intron is not fully understood.