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Addgene

HIR WT
(Plasmid #24049)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24049 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRT3
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Insulin Receptor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4600
  • Entrez Gene
    INSR (a.k.a. CD220, HHF5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HIR WT was a gift from Frederick Stanley (Addgene plasmid # 24049 ; http://n2t.net/addgene:24049 ; RRID:Addgene_24049)

  • For your References section:

    Insulin receptor tyrosine kinase activity and phosphorylation of tyrosines 1162 and 1163 are required for insulin-increased prolactin gene expression. Jacob KK, Whittaker J, Stanley FM. Mol Cell Endocrinol. 2002 Jan 15. 186(1):7-16. 10.1016/S0303-7207(01)00674-8 PubMed 11850117
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