KAP123-FLU (116)
(Plasmid
#24048)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYEX-U (modified pYEX-BX)
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Backbone manufacturerRosemary Williams (Rout Lab)
- Backbone size w/o insert (bp) 7100
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKAP123
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneKAP123 (a.k.a. YER110C, YRB4)
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Tags
/ Fusion Proteins
- FLU (C terminal on insert)
- FLU (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer yCUP1pro-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
KAP123 fused upstream of two FLU tags in BamHI/SalI of pBT024
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KAP123-FLU (116) was a gift from Michael Rout (Addgene plasmid # 24048 ; http://n2t.net/addgene:24048 ; RRID:Addgene_24048)