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Addgene

KAP121-FLU (115)
(Plasmid #24047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYEX-U (modified pYEX-BX)
  • Backbone manufacturer
    Rosemary Williams (Rout Lab)
  • Backbone size w/o insert (bp) 7100
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    KAP121
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    PSE1 (a.k.a. YMR308C, KAP121)
  • Tags / Fusion Proteins
    • FLU (C terminal on insert)
    • FLU (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer yCUP1pro-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KAP121 fused upstream of two FLU tags in BamHI/SalI of pBT024

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KAP121-FLU (115) was a gift from Michael Rout (Addgene plasmid # 24047 ; http://n2t.net/addgene:24047 ; RRID:Addgene_24047)
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