RPL3-GFP (96)
(Plasmid
#24040)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneyEGFP3
- Backbone size w/o insert (bp) 5000
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRPL3
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneRPL3 (a.k.a. YOR063W, MAK8, TCM1, YOR29-14)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Entire RPL3 ORF inserted upstream of eGFP in EcoRI/HindIII of yEGFP3.
An F379S mutation found in Addgene quality control sequencing. No evidence to suggest mutation affects activity.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RPL3-GFP (96) was a gift from Michael Rout (Addgene plasmid # 24040 ; http://n2t.net/addgene:24040 ; RRID:Addgene_24040)