RPL25NLS-GFP (101)
(Plasmid
#24038)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24038 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYX242
- Backbone size w/o insert (bp) 5000
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRPL25
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)135
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MutationOnly bp 1-135 (aa 1-45) of RPL25
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Entrez GeneRPL25 (a.k.a. YOL127W)
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
NLS of RPL25 (bp 1-135) fused upstream of eGFP in EcoRI/SalI of pYX242
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RPL25NLS-GFP (101) was a gift from Michael Rout (Addgene plasmid # 24038 ; http://n2t.net/addgene:24038 ; RRID:Addgene_24038)