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Addgene

pMOTag2T-crelox-HYG
(Plasmid #24025)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24025 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMOTag
  • Backbone size (bp) 5414
  • Vector type
    Cre/Lox
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMOTag2T-crelox-HYG made by Jenny Li taking the HindIII-BamHI crelox-HYG fragment from pyrFEKO-HYG and inserting into HindIII-BamHI digested pMOTag2T made by Luisa.
Based on pMOTag series made in pBluescript II KS+

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMOTag2T-crelox-HYG was a gift from George Cross (Addgene plasmid # 24025 ; http://n2t.net/addgene:24025 ; RRID:Addgene_24025)
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