pLEW100cre_del_tetO
(Plasmid
#24019)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24019 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEW100cre
- Backbone size (bp) 6598
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Vector typeTrypanosome Transient Expression
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Selectable markersPhleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Cre Recombinase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A
- 3′ sequencing primer CRE-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pLEW100cre with the tet operator deleted so no need for tetracycline induction. This is the plasmid to use for transient cre expression in cells that express the tet repressor
For more information see: http://tryps.rockefeller.edu/trypsru2_cre-lox.html
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEW100cre_del_tetO was a gift from George Cross (Addgene plasmid # 24019 ; http://n2t.net/addgene:24019 ; RRID:Addgene_24019)
Map uploaded by the depositor.
