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Addgene

pLEW100v5-177-HYG
(Plasmid #24013)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEW100
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Trypanosome Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase
  • Insert Size (bp)
    2000
  • Mutation
    K520R
  • GenBank ID
    AAK13426

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T7 terminators added: regulated rRNAp drives luciferase, giving about 10-fold higher te-induced expression in BF than pLEW100 with no increase in background.

Target locus: 177-bp rep

Regulation: 2 tet Op 2 T7 terminators

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEW100v5-177-HYG was a gift from George Cross (Addgene plasmid # 24013 ; http://n2t.net/addgene:24013 ; RRID:Addgene_24013)
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