pLEW82v4
(Plasmid
#24009)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEW82
- Backbone size w/o insert (bp) 4000
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Vector typeTrypanosome Expression
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Selectable markersPhleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLuciferase
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Insert Size (bp)2000
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GenBank IDAAK13426
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Added a constitutive 10% T7p to drive BLE, 3 Tet operators to the T7 promoter, and revised sites for cloning reporter genes. High expression poor regulation.
Target locus: rRNA spacer
Regulation: 3 tet Op 2 T7 terminators
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEW82v4 was a gift from George Cross (Addgene plasmid # 24009 ; http://n2t.net/addgene:24009 ; RRID:Addgene_24009)
Map uploaded by the depositor.
