pLEW13
(Plasmid #24007)

• Depositing Lab: George Cross
• Publication: George Cross Lab Plasmids (unpublished)

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 6000
• Vector type: Trypanosome Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: T7 RNA Polymerase
• Insert Size (bp): 3000

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A
• 3′ sequencing primer: Neo-R

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

T7 RNA Polymerase and Tet Repressor present (see map).

Target locus: Tubulin

Regulation: None