pCI-SEP-GluR2(Q)
(Plasmid #24002)

• Depositing Lab: Robert Malinow
• Publication: Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Backbone

• Vector backbone: pCI
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GluR2
• Species: R. norvegicus (rat)
• Mutation: R607Q
• Entrez Gene: Gria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GluR2 signal peptide (aa1-24) (N terminal on insert)
  • SEP (superecliptic pHluorin) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: EBV-rev

Resource Information

• Supplemental Documents:
  • Annotated Genbank file
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry