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Addgene

pCI-SEP GluR2(R)
(Plasmid #24001)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24001 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCI
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GluR2
  • Species
    R. norvegicus (rat)
  • Entrez Gene
    Gria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GluR1 signal peptide (aa1-22) (N terminal on insert)
    • SEP (superecliptic pHluorin) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-SEP GluR2(R) was a gift from Robert Malinow (Addgene plasmid # 24001 ; http://n2t.net/addgene:24001 ; RRID:Addgene_24001)
  • For your References section:

    Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. Kopec CD, Li B, Wei W, Boehm J, Malinow R. J Neurosci. 2006 Feb 15. 26(7):2000-9. 10.1523/JNEUROSCI.3918-05.2006 PubMed 16481433

Ready-to-use antibodies

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