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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 24001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGluR2
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SpeciesR. norvegicus (rat)
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Entrez GeneGria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
- Promoter CMV
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Tags
/ Fusion Proteins
- GluR1 signal peptide (aa1-22) (N terminal on insert)
- SEP (superecliptic pHluorin) (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-SEP GluR2(R) was a gift from Robert Malinow (Addgene plasmid # 24001 ; http://n2t.net/addgene:24001 ; RRID:Addgene_24001) -
For your References section:
Glutamate receptor exocytosis and spine enlargement during chemically induced long-term potentiation. Kopec CD, Li B, Wei W, Boehm J, Malinow R. J Neurosci. 2006 Feb 15. 26(7):2000-9. 10.1523/JNEUROSCI.3918-05.2006 PubMed 16481433