pCI-SEP_NR2B
(Plasmid #23998)

• Depositing Lab: Robert Malinow
• Publication: Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Backbone

• Vector backbone: pCI
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NR2B
• Species: R. norvegicus (rat)
• Insert Size (bp): 6300
• GenBank ID: EDM01617.1
• Entrez Gene: Grin2b (a.k.a. GluN2B)
• Promoter: CMV
• Tags / Fusion Proteins:
  • NR2B signal sequence (aa1-31) (N terminal on backbone)
  • SEP (superecliptic pHluorin) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See attached map for additional plasmid features.
The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.