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Addgene

pCI-SEP_NR2A Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme(s): XhoI, BamHI and XhoI+BamHI double digest.

XhoI linearized the plasmid at ~12kb; BamHI cuts the plasmid twice giving a ~5.3kb+7kb band; a double digest results in 7kb+3.7kb+1.6kb bands.