pCI-SEP_NR2A
(Plasmid #23997)

• Depositing Lab: Robert Malinow
• Publication: Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Backbone

• Vector backbone: pCI
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 12000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM109
• Growth instructions: JM109 Cells
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NR2A
• Species: R. norvegicus (rat)
• Insert Size (bp): 5800
• Entrez Gene: Grin2a (a.k.a. GluN2A, NMDAR2A, NR2A)
• Tag / Fusion Protein:
  • SEP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See attached map for additional plasmid features.
The super-ecliptic pHluorin (SEP) coding sequence was inserted three amino acids downstream of the predicted signal peptide cleavage site.