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Addgene

pTOPO-25S rDNA
(Plasmid #23996)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23996 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Bacterial Cloning - TOPO

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    TOP10 or other E. coli strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    25S rRNA from +5270 to oligo y
  • Alt name
    25S rDNA from +5270 to oligo y
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    200
  • Mutation
    none
  • GenBank ID
    FN393078

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTOPO-25S rDNA was a gift from Susan Baserga (Addgene plasmid # 23996 ; http://n2t.net/addgene:23996 ; RRID:Addgene_23996)

  • For your References section:

    RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL, Baserga SJ. Genes Dev. 2004 Oct 15. 18(20):2506-17. 10.1101/gad.1226604 PubMed 15489292
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