pTOPO-rDNA-NTS
(Plasmid
#23992)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23992 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Cloning - TOPO
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsTOP10 or other E. coli strain
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerDNA NTS
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Alt namerDNA non-transcribed spacer
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SpeciesS. cerevisiae (budding yeast)
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MutationNone
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GenBank IDFN393078
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTOPO-rDNA-NTS was a gift from Susan Baserga (Addgene plasmid # 23992 ; http://n2t.net/addgene:23992 ; RRID:Addgene_23992) -
For your References section:
RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Gallagher JE, Dunbar DA, Granneman S, Mitchell BM, Osheim Y, Beyer AL, Baserga SJ. Genes Dev. 2004 Oct 15. 18(20):2506-17. 10.1101/gad.1226604 PubMed 15489292