VCP(DK0)-EGFP
(Plasmid #23974)

• Depositing Lab: Nico Dantuma
• Publication: Tresse et al Autophagy. 2010 Feb 22. 6(2):.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Valosin containing protein
• Alt name: p97
• Alt name: CDC48
• Alt name: TERA
• Species: H. sapiens (human)
• Insert Size (bp): 2419
• Mutation: E305QE578Q
• Entrez Gene: VCP (a.k.a. CDC48, FTDALS6, TERA, p97)
• Tags / Fusion Proteins:
  • EGFP (C terminal on backbone)
  • 6xHis (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry