pTreTight-Htt94Q-CFP
(Plasmid #23966)

• Depositing Lab: Nico Dantuma
• Publication: Maynard et al Proc Natl Acad Sci U S A. 2009 Aug 18. 106(33):13986-91.

Backbone

• Vector backbone: pTRE-tight
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 2605
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: N-terminal fragment Huntingtin Q94
• Alt name: Htt
• Alt name: HttQ94
• Alt name: Htt polyQ
• Species: H. sapiens (human)
• Mutation: 94 polyQ repeat
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Tag / Fusion Protein:
  • CFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest for 23966
• Articles Citing this Plasmid:
  • 12 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are 96Q repeats and an Argenine in the middle of the repeat.