Zahra78-79
(Plasmid #23960)

• Depositing Lab: Lawrence Cohen
• Publication: Lawrence Cohen lab plasmids (unpublished)

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VSP
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2000
• Mutation: R153Q T156R
• Entrez Gene: tpte (a.k.a. tpip, vsp, wu:fd20e11, wu:fi24b06)
• Tags / Fusion Proteins:
  • Citrulline
  • Cerulean

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry