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Addgene

pEV-p105-WT
(Plasmid #23952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 23952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pEVRF (pLG-033)
  • Backbone manufacturer
    Ghost lab
  • Backbone size w/o insert (bp) 5120
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p105
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2916
  • Entrez Gene
    Nfkb1 (a.k.a. NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105)
  • Tag / Fusion Protein
    • T7 gp10 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEV-p105-WT was a gift from Warner Greene (Addgene plasmid # 23952 ; http://n2t.net/addgene:23952 ; RRID:Addgene_23952)

  • For your References section:

    Cotranslational biogenesis of NF-kappaB p50 by the 26S proteasome. Lin L, DeMartino GN, Greene WC. Cell. 1998 Mar 20. 92(6):819-28. 10.1016/S0092-8674(00)81409-9 PubMed 9529257
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